High frequency shoot regeneration from trifoliate orange ( Poncirus trifoliata L. Raf.) using the thin cell layer method
Identifieur interne : 000208 ( France/Analysis ); précédent : 000207; suivant : 000209High frequency shoot regeneration from trifoliate orange ( Poncirus trifoliata L. Raf.) using the thin cell layer method
Auteurs : Bui Van Le [France] ; N. Thanh Ha [Viêt Nam] ; L. T Anh Hong [Viêt Nam] ; Kim Trân Thanh Vân [France]Source :
- Comptes Rendus de l'Academie des Sciences Series III Sciences de la Vie [ 0764-4469 ] ; 1999.
English descriptors
Abstract
A method for a high frequency and direct in vitro bud regeneration of a woody species, the trifoliate orange (Poncirus trifoliata L. Raf), was designed. Transverse thin cell layer (tTCL) explants excised from the stem internodes of 1-year-old young plants of P. trifoliata regenerated bud in vitro on a medium containing 6-benzylaminopurine (BAP 1-50 μM) and N-phenyl-N'-1,2,3-thidiazol-5-ylurea (thidiazuron, TDZ) (0.1–10 μM). The optimal concentrations for bud induction were 25 μM BAP and 1 μM TDZ leading to 87 and 72 % of responsive tTCLs and 24 and 15 buds per tTCL, respectively. A higher percentage of responsive tTCLs and a higher frequency of bud regeneration were obtained with BAP and TDZ combined. With a combination of 10 μM BAP and 1 μM TDZ, 90 % of responsive tTCLs forming 37 buds per tTCL were obtained. Shoot elongation occurred after a transfer onto a medium containing 1 μM GA3. Rooting of individual shoot was induced using 5 μM NAA. One hundred per cent of rooted shoots developed normally after transfer to the greenhouse; no phenotype variation was observed. High numbers of regenerated viable plants can be produced directly without callus formation from tTCL after 9 weeks of culture.
Url:
DOI: 10.1016/S0764-4469(99)00110-9
Affiliations:
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T Anh Hong</name></author><author><name sortKey="Tran Thanh Van, Kim" sort="Tran Thanh Van, Kim" uniqKey="Tran Thanh Van K" first="Kim" last="Trân Thanh Vân">Kim Trân Thanh Vân</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1A72EB57866F1BF873707228F48C4F6150EF2A31</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0764-4469(99)00110-9</idno><idno type="url">https://api.istex.fr/document/1A72EB57866F1BF873707228F48C4F6150EF2A31/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000131</idno><idno type="wicri:Area/Istex/Curation">000131</idno><idno type="wicri:Area/Istex/Checkpoint">000B64</idno><idno type="wicri:doubleKey">0764-4469:1999:Van Le B:high:frequency:shoot</idno><idno type="wicri:Area/Main/Merge">003315</idno><idno type="wicri:Area/Main/Curation">003090</idno><idno type="wicri:Area/Main/Exploration">003090</idno><idno type="wicri:Area/France/Extraction">000208</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">High frequency shoot regeneration from trifoliate orange ( Poncirus trifoliata L. 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Raf), was designed. Transverse thin cell layer (tTCL) explants excised from the stem internodes of 1-year-old young plants of P. trifoliata regenerated bud in vitro on a medium containing 6-benzylaminopurine (BAP 1-50 μM) and N-phenyl-N'-1,2,3-thidiazol-5-ylurea (thidiazuron, TDZ) (0.1–10 μM). The optimal concentrations for bud induction were 25 μM BAP and 1 μM TDZ leading to 87 and 72 % of responsive tTCLs and 24 and 15 buds per tTCL, respectively. A higher percentage of responsive tTCLs and a higher frequency of bud regeneration were obtained with BAP and TDZ combined. With a combination of 10 μM BAP and 1 μM TDZ, 90 % of responsive tTCLs forming 37 buds per tTCL were obtained. Shoot elongation occurred after a transfer onto a medium containing 1 μM GA3. Rooting of individual shoot was induced using 5 μM NAA. One hundred per cent of rooted shoots developed normally after transfer to the greenhouse; no phenotype variation was observed. High numbers of regenerated viable plants can be produced directly without callus formation from tTCL after 9 weeks of culture.</div></front></TEI><affiliations><list><country><li>France</li><li>Viêt Nam</li></country><region><li>Île-de-France</li></region><settlement><li>Orsay</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Van Le, Bui" sort="Van Le, Bui" uniqKey="Van Le B" first="Bui" last="Van Le">Bui Van Le</name></region><name sortKey="Tran Thanh Van, Kim" sort="Tran Thanh Van, Kim" uniqKey="Tran Thanh Van K" first="Kim" last="Trân Thanh Vân">Kim Trân Thanh Vân</name></country><country name="Viêt Nam"><noRegion><name sortKey="Thanh Ha, N" sort="Thanh Ha, N" uniqKey="Thanh Ha N" first="N" last="Thanh Ha">N. Thanh Ha</name></noRegion><name sortKey="Anh Hong, L T" sort="Anh Hong, L T" uniqKey="Anh Hong L" first="L. T" last="Anh Hong">L. 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